Full Journey

Gene Knockdown via siRNA In-Vitro

How do we reduce the expression of a specific gene inside the cell to study its function? This course is a complete learning journey in three stages: Stage 1: The Lecture The principle of transient gene silencing mediated by siRNA How synthetic siRNA duplexes are delivered into cells using lipid-based transfection reagents The role of the RISC complex in recognizing and degrading complementary mRNA Applications of the technique in studying gene function, disease mechanisms, and target validation in drug discovery Stage 2: Lab Preparation Preparing cultured cells, transfection reagents, and siRNA duplexes Safe handling of reagents and avoiding factors that reduce transfection efficiency Stage 3: Hands-On Workshop Performing the transfection step by step: preparing the siRNA-lipid complex and adding it to the cells Assessing knockdown efficiency and comparing target gene expression against the control group