Courses
Learn Lab Techniques
Video courses delivered by experts so you can master lab techniques. Watch, learn, and practice at your own pace with lifetime access.
IntermediateGene Knockdown Via siRNA In-Vitro
siRNA-mediated gene knockdown is a transient gene-silencing technique that reduces expression of a specific gene in cultured cells. Synthetic siRNA duplexes are delivered into cells using a lipid-based transfection reagent and loaded into the RISC complex, which recognizes and degrades complementary mRNA. The method is widely used to study gene function, disease mechanisms, and target validation in drug discovery.
IntermediateRNA Extraction From Cultured Cells
RNA extraction is a liquid-phase separation technique used to isolate high-quality total RNA from cultured cells. Using TRIzol reagent, cellular components are lysed and separated into aqueous, interphase, and organic layers. RNA is then precipitated, washed, and dissolved for downstream molecular applications such as cDNA synthesis, RT-PCR, qPCR, RNA sequencing, and RNase protection assays.
IntermediateMolecular Genetics Laboratory Techniques: PCR To Next-Generation Sequencing
A hands-on advanced course covering the core wet-lab and analytical workflows behind modern molecular genetics. Learners move through nucleic acid extraction and quality control, conventional and quantitative PCR, gel and capillary electrophoresis, Sanger sequencing, and the full next-generation sequencing pipeline — library preparation, target enrichment, sequencing chemistry, and variant calling. The course pairs technique theory with troubleshooting (contamination, primer-dimers, low yield, batch effects) and covers good laboratory practice, controls, and result validation. By the end, participants can select the right technique for a research or diagnostic question, run it correctly, and critically interpret the output.
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