Course
siRNA-mediated gene knockdown is a transient gene-silencing technique that reduces expression of a specific gene in cultured cells. Synthetic siRNA duplexes are delivered into cells using a lipid-based transfection reagent and loaded into the RISC complex, which recognizes and degrades complementary mRNA. The method is widely used to study gene function, disease mechanisms, and target validation in drug discovery.
Chapter 01
Introduction
Introduction
7:06Chapter 02
Experiment Materials
3:46Chapter 03
Cell cultures reagents
Chapter 04
The transfection reagent
2:36This course includes a hands-on lab simulation — unlocked free when you buy the course.
Chapter 05
The practical part
14:10Chapter 06
Tools for assessing your gene knockdown
1:58Chapter 07
Troubleshooting and conclusion
2:41
