• Real-time PCR
Real-time PCR

Real-time PCR

CATEGORY

Genomics

FEE

39.90 SAR

DURATION

5 Day

About Course

A. RNA extraction: RNA extraction is a fundamental technique in molecular biology, crucial for studying gene expression and understanding cellular functions. This experiment aims to isolate RNA from a biological sample, enabling subsequent analyses such as quantitative PCR (qPCR) and RNA sequencing. B. cDNA Synthesis: cDNA (complementary DNA) synthesis is the process of creating a DNA copy from an RNA template. This is crucial because DNA is more stable and easier to work with than RNA for many molecular biology techniques. The enzyme reverse transcriptase is used to transcribe RNA into cDNA. This process enables researchers to study gene expression by converting RNA, which reflects active genes, into a stable DNA form. C. Polymerase Chain Reaction (PCR): PCR is a technique used to amplify specific DNA sequences. It’s akin to photocopying a particular section of a book repeatedly. PCR involves three main steps: 1. Denaturation: heating the DNA to separate its two strands. 2. Annealing: cooling the mixture to allow primers to bind to their complementary sequences on the DNA. 3. Extension: using the enzyme DNA polymerase to extend the primers and synthesize new DNA strands.

Requirements

  • Basic understanding of Genomics
  • Commitment to 5 days of learning
  • Access to a computer or mobile device

How to Apply

  • Visit the course registration page and fill out your details
  • Make the payment for the course
  • You will receive a confirmation email with further details

Fees and Funding

Fees are 39.90 SAR. Additional funding options may be available for eligible candidates.

Instructor
Abduallah

Abduallah Alhamed

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